43 DB43/T2174—2021 湖南省地方标准 辣椒白绢病抗性室内鉴定技术规程 Rulers for Evaluation of pepper for resistance to Southern BlightICS CCS 67.080.20 B 31 发 布 湖南省市场监督管理局2021-08发布-29 2021-10实施-29 DB43/T 2174—2021 I 目 次 前言 ································ ································ ································ ························ Ⅲ 1 范围 ································ ································ ································ ····················· 1 2 术语和定义 ································ ································ ································ ············ 1 2.1 辣椒白绢病 Pepper Southern Blight ································ ································ ·· 1 2.2 辣椒白绢病抗性 ································ ································ ································ 1 3 白绢病菌接种体制备 ································ ································ ································ 1 3.1 病原物分离与鉴定 ································ ································ ····························· 1 3.2 接种体制备 ································ ································ ································ ······ 1 3.3 培养基配制 ································ ································ ································ ······ 1 3.4 病原物保存 ································ ································ ································ ······ 2 4 白绢病抗性室内鉴定 ································ ································ ································ 2 4.1 鉴定室 ································ ································ ································ ············ 2 4.2 鉴定材料育苗 ································ ································ ································ ··· 2 4.3 接种时期 ································ ································ ································ ········· 2 4.4 接种方法 ································ ································ ································ ········· 2 4.5 接种后管理 ································ ································ ································ ······ 2 5 病情鉴定 ································ ································ ································ ··············· 2 5.1 病情级别划分 ································ ································ ································ ··· 2 5.2 调查方法 ································ ································ ································ ········· 3 6 抗病性评价 ································ ································ ································ ············ 3 6.1 抗病性评价标准 ································ ································ ································ 3 6.2 鉴定结果的有效性判别 ································ ································ ······················· 3 7 记录与保存 ································ ································ ································ ············ 3 附录A（资料性） 辣椒白绢病病原菌的学名、形态描述和分子鉴定 ································ ······ 5 DB43/T 2174—2021 II DB43/T 2174—2021 III 前 言 本文件按照 GB/T 1.1 —2020《标准化工作导则 第1部分：标准化文件的结构和起草规则》的规 定起草。 请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。 本标准由湖南省农业农村厅提出。 本标准由湖南省标准 化技术委员会归口。 本标准主要起草单位：湖南 省蔬菜研究所、湖南农业大学植物保护学院、湖南省微生物研究院、湖 南省植物保护研究所、湖南大学研究生院隆平分院、衡南县植保植检站。 本文件主要起草人：郑井元、吴永红、周书栋、李雪峰、陈武、刘清术、朱春晖、文湘钰、张纯、 王俊莹、张竹青、陈文超、杨莎、黄军、李伟兵。 DB43/T 2174—2021 IV DB43/T 2174—2021 1 辣椒白绢病抗性室内鉴定技术规程 1 范围 本文件规定了辣椒白绢病抗性鉴定的定义和术语、接种体制备、室内抗性鉴定、病情调查、抗病性 评价以及记录与保存。 本文件适用于湖南省或相似地区辣椒 种质资源白绢病抗性室内鉴定。 2 术语和定义 下列术语和定义适用于本文本。 2.1 辣椒白绢病 Pepper Southern Blight 由齐整小核菌（ Sclerotium rolfsii.sacc ）侵害植株茎基部和根部引起的土传性病害。发病时近地面茎 基部初呈浅褐色水浸状腐烂，渐变成深褐色，病部凹陷，表面产生白色绢丝状菌丝，集结成束，呈辐射 状向茎上发展，并向茎基部四周地面扩展，病健部分界明显，在茎基部近土表有白色霉层产生，菌丝向 左右扩大后绕茎一周，地上部叶片迅速萎蔫，叶色变黄变淡，整株枯死。 2.2 辣椒白绢病抗性 辣椒在白绢病菌的侵染下， 能尽量减少损害， 或受到侵染后能恢复生长， 继续保持生命活力的特征。 3 白绢病菌接种体制备 3.1 病原物分离与鉴定 从发病田间采集具有典